The dependency on beta 2 integrins in neutrophil recruitment to the skin in the Arthus reaction was examined using alpha L, alpha M and beta 2 knockout mice. Then, we evaluated the effect of isoflurane on neutrophil recruitment to the skin. In addition, the effects of isoflurane on neutrophil
binding to intercellular adhesion molecule-1 (ICAM-1), one of the beta 2 integrin ligands, were studied in vitro using cell adhesion assays.
Neutrophil recruitment to the skin in the Arthus reaction model was totally dependent on beta 2 integrins, as beta 2 knockout mice completely abolished it. However, the defect of only one of the beta 2 integrins was not sufficient to abolish neutrophil recruitment. Isoflurane reduced neutrophil
recruitment to the skin by approximately 90 %. Also, isoflurane inhibited neutrophil adhesion to beta 2 integrin ligand LY3023414 chemical structure ICAM-1.
We demonstrated that (1) neutrophil recruitment to the skin was totally dependent on beta 2 integrins, and (2) isoflurane significantly impaired neutrophil recruitment. Based on the previous studies on the contribution of other adhesion molecules in neutrophil recruitment, it is likely that isoflurane at least partially affects on beta 2 integrins in this model.”
“VP7 is a major group-specific protein of the bluetongue virus (BTV), and is therefore a candidate for use as a diagnostic reagent. In this study, BALB/c mice were immunized with BTV16, and the lymphocyte hybridoma technique and indirect ELISA screening method were employed to obtain two U0126 strains of hybridoma cells secreting specific monoclonal antibodies (MAbs) to BTV16. Eukaryotic recombinant
plasmids coding for 10 segments of BTV16 separately were transfected into BHK-21 cells, respectively, followed by immunofluorescence, showing that two MAbs only reacted with BTV-VP7. Western blot analysis showed the same result. Indirect immunofluorescence results indicated that two of the MAbs present different response spectrums with BTV1 similar to 24 serotypes. These results indicate that these MAbs may be good candidates for a specific diagnostic method and functional exploration of the VP7 protein.”
“Aim: To disclose GSK3326595 concentration potential risk factors for sustaining a fractured clavicle in the newborn.
Method: A retrospective case-control analysis of women who gave birth to an infant with a fractured clavicle during a four-year period (2003-2006) was performed. A control group of newborns who did not sustain a fractured clavicle was formed (2: 1) matched for maternal age, parity and gestational age at delivery.
Results: The rate of fractured clavicle was 0.35%. Heavier newborns’ birth weight (3632.9 +/- 376.1 g vs 3429.5 +/- 513.0 g, P < 0.05) and the use of oxytocin (91.3% vs 69.5%, P < 0.05) were associated with the occurrence of fractured clavicle during birth.