Unlike colicin Ia- and microcin V-encoding determinants , pColE1 was independently associated with pColIa in the UTI strains. Thus, colicin E1 itself appears to be a potentially important virulence factor of certain uropathogenic strains of E. coli. Methods Combretastatin A4 bacterial strains Altogether, 772 human E. coli strains were isolated between May 2007 and June 2009, from both male and female patients. Five hundred and fifty-nine strains were collected from the Faculty Hospital Bohunice, Brno, CZ, including
361 E. coli strains isolated from urinary tract infections (UTI) and 198 E. coli strains isolated from feces of patients without bacterial gut infections (control commensal strains). Additional 213 strains of E. coli (isolated from feces of patients without bacterial gut infections) were collected from SAHA HDAC research buy the St. Ann’s Faculty Hospital, Brno, CZ. Out of 411 E. coli control strains (190 of male and 221 of female origin), only 92 (22.4%) stemmed from patients with primary diagnoses related to the gastrointestinal system (e.g. pancreatitis, BI 10773 chemical structure dyspepsia etc.) and none were isolated from cases with detectable bacterial intestinal infection. Since no statistically significant differences in the incidence of producer strains or the incidence of individual bacteriocin types between control groups from both hospitals were found, strains from both groups were merged and treated as a single group. UTI strains
were isolated from 85 males and 276 females. Bacterial identification of E. coli was performed using a set of biochemical reactions (ENTEROtest 16, PLIVA-Lachema Diagnostika, Czech Republic). All Phosphatidylethanolamine N-methyltransferase donors of investigated strains were Caucasians living in the South Moravia region of the Czech Republic. For each sample, the primary diagnosis of the source patient was established by an experienced clinician. A described set of E. coli indicator strains was used to identify the colicin and microcin types produced: E. coli K12-Row, C6 (ϕ), B1, P400, and Shigella sonnei 17 ; additionally,
one recently verified indicator strain, E. coli S40, was also used . Together, these indicator strains are capable of detecting all known colicin types including colicin L (P400) and colicin Js (S.s. 17). Control bacterial producers encoding different colicin types were taken from laboratory stock and comprised E. coli BZB2101pColA – CA31, BZB2102 pColB – K260, BZB2103 pColD – CA23, BZB2107 pColE4 – CT9, BZB2108 pColE5 – 099, BZB2150 pColE6 – CT14, BZB2120 pColE7 – K317, BZB2279 pColIa – CA53, BZB2202 ColIb – P9, BZB2116 pColK – K235, PAP1 pColM – BZBNC22, BZB2123 pColN – 284 (original source: A. P. Pugsley), E. coli 189BM pColE2 – P9 (B. A. D. Stocker), E. coli 385/80 pColE1, pColV (H. Lhotová), E. coli 185M4 pColE3 – CA38 (P. Fredericq), E. coli W3110 pColE8, W3110 pColE9 (J. R. James), E. coli K-12 pColS4 (D. Šmajs), S. boydii M592 (serovar 8) pColU (V. Horák), E. coli K339 pColY (D.