This was similar for SGII salivary spacers (45% persistent in Subject #1, 65% in Subject #2, 51% in Subject #3, and 58% in Subject #4) (Additional file JIB04 supplier 2: Figure S3 and Additional file 1: Table S4). There was a smaller yet similar group of spacers on the skin of each subject for SGI spacers (38% in Subject #1, 36% in Subject #2, 15% in Subject #3, and 24% in Subject #4) and SGII spacers (39% in Subject #1, 28% in Subject #2, 10% in Subject #3, and 36% in Subject #4) persisting throughout the study. Many of the conserved spacers in saliva matched spacers on the skin of each subject for SGI spacers (44% in Subject #1, 41% in Subject #2,

11% in Subject #3, and 25% in Subject #4) and SGII spacers (42% in Subject #1, 30% in Subject #2, 17% in Subject #3, and 37% in Subject #4). Figure 1 Heatmaps of SGI CRISPR spacer groups in all subjects. Each row represents a unique spacer group and the columns represent each

individual time point. Each day is listed, where M represents morning, N represents noon, and E represents evening. Saliva-derived SGI CRISPR spacer groups are demonstrated on the left, and skin-derived CRISPR spacer groups are on the right of each panel. The intensity scale bar is located to the right, and represents the percentage of total spacers found at each time point in each subject. Panel A – Subject #1, Panel B – Subject #2, Panel C – Subject #3, and Panel D – Subject #4. Figure 2 SGI CRISPR spacer BTK signaling pathway inhibitor group heat matrices from all subjects. Each matrix demonstrates the percentage

of shared SGI CRISPR spacer groups between all time points within each subject. The top DMXAA chemical structure triangular portion of each matrix represents comparisons between saliva-derived CRISPR spacers, the bottom rectangular portion of each matrix represents comparisons between saliva-derived and skin-derived CRISPR spacers, and the bottom triangular portion of each matrix represents comparisons between skin-derived CRISPR spacers. The intensity scale bar is located to the right of each matrix. Panel PJ34 HCl A – Subject #1, Panel B – Subject #2, Panel C – Subject #3, and Panel D – Subject #4. We measured the relative conservation of SGII and SGI spacers by time of day sampled to determine whether there were biases in CRISPR spacer profiles on the skin and in the saliva based on sampling times. We found that in the saliva, there was significantly greater conservation (p < 0.05) of CRISPR spacer profiles in the AM for both SGII (Figure 3, Panel A) and SGI spacers (Panel B). Similar conservation of CRISPR spacer profiles were not found for Noon and PM time points for either SGII or SGI spacers in saliva (Additional file 2: Figures S4 and S5).