For this a dose of 19 mGy/min was measured, resulting in 202 mGy/scan [11]. Animals received between 4 and 15 repetitive exams with 4 weeks interscan interval (MV = 13.0, SD = 3.05). The Vactosertib in vivo calculated accumulative dose ranged from 808 mGy within 91 days (4 exams) to 3030 mGy within 475 d (15 exams). The mean calculated accumulative dose was 2626 mGy within approximately 450 d. These dose values in synopsis with a reported LD50/30 (dose PLX-4720 chemical structure that is lethal in 50% of the animals within 30 days) of 7.52 Gy demonstrate the relevance of the issue [24]. However, we consider direct adverse effects (structural changes to the lungs or unintended radiation effects on the tumour growth) to be unlikely.

Although gene expression changes have been seen in cell cultures with doses as low as 20-500 mGy [25] structural changes like fibrosis were not even seen following doses as high as 7-9 Gy [24] and the reported RGFP966 datasheet values for therapeutic radiation also amounted to values as high as 15.5 Gy [12]. In conclusion the presented region-growing segmentation algorithm allows longitudinal in-vivo quantification of multifocal lung adenocarcinoma in SPC-raf transgenic mice. This enables the assessment of tumor load and growth kinetics for the study of carcinogenesis and the evaluation of novel treatment strategies. Acknowledgements The publication of this study is supported by the German Research Foundation (DFG)-project

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