Alternatively, the corresponding regression equations were derived. Preparation of tolperisone hydrochloride and Ganetespib Phase 3 etodolac test solution Twenty tablets were weighed accurately, taking an equivalent of 15 mg of TOLP : 40 mg of ETD tablet powder, and transferred to a 100 ml volumetric flask. This was sonicated with 60 ml methanol for 20 minutes and the volume was made up to 100 ml with the same solvent. This solution was filtered through a milipore filter. Four milliliters of this solution was transfered to a 100 ml volumetric flask and the volume was made up using the mobile phase. Validation The method was validated for assay of TOLP and ETD in accordance with ICH guidelines.[16] Linearity In order to check the linearity for the developed method, solutions of six different concentrations ranging from 3.
0 �C 21.0 ��g / ml were prepared for TOLP and 8 �C 56 ��g / ml for ETD, respectively. The chromatograms were recorded and the peak areas are given in Table 1. A linear relationship between areas versus concentrations was observed in the above-mentioned linearity range. This range was selected as the linear range for the development of the analytical method, for the estimation of TOLP and ETD. Table 1 Linearity data for tolperisone hydrochloride and etodolac Sensitivity The sensitivity of the measurement of TOLP and ETD using the proposed method was estimated as the limit of quantification (LOQ) and the lowest concentration detected under these chromatographic conditions as the limit of detection (LOD). The LOD and LOQ were calculated by using the equations LOD = 3.
3 �� �� / S and LOQ = 10 �� �� / S, where �� was the standard deviation of the peak areas of the drug (n = 6), and S was the slope of the corresponding calibration plot. The limits of detection and quantification for TOLP were 0.16 ��g / ml and 0.51 ��g / ml, respectively, and those for ETD were 0.58 ��g / ml and 1.7 ��g / ml, respectively. System suitability Various system suitability parameters were also calculated. It was observed that all the values were within the limits, and are shown in Table 2. The statistical evaluation of the proposed method revealed its good linearity, reproducibility, and its validation of different parameters and led us to the conclusion that it could be used for the rapid and reliable determination of TOLP and ETD in tablet formulation.
The results are furnished in Table 2. Table 2 System suitability parameters for tolperisone hydrochloride and etodolac Precision Precision was measured by the analysis of sample solutions three times at three different concentrations. Anacetrapib Solutions containing 3, 6, and 9 ��g / ml of TOLP and 8, 16, and 24 ��g / ml of ETD were subjected to the proposed HPLC analysis, to check the intraday and interday variations of the method. The results are furnished in Tables Tables33 and and44.