(XLS 91 KB) Additional file 3: Table S3. Fumarate reductase activity under anaerobic conditions. This file contains BIBF 1120 cost the specific activity of fumarate reductase in cell-free extracts isolated from 14028s and Δfur under anaerobic conditions. (PDF 143 KB) Additional file 4: Table S4. Genes regulated by Fur and Fnr under anaerobiosis and contain putative binding sites for both regulators. This file contains genes that were differentially expressed in 14028s, Δfur, and the fnr, which contain a putative

binding site for Fur and for Fnr. (PDF 23 KB) References 1. Lee JW, Helmann JD: Functional specialization within the Fur family of metalloregulators. Biometals 2007,20(3–4):485–499.PubMedCrossRef 2. Bagg A, Neilands JB: Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor selleck to bind the operator of an iron transport operon in Escherichia coli . Biochemistry 1987,26(17):5471–5477.PubMedCrossRef 3. Neilands JB: Siderophores. Arch Biochem Biophys 1993,302(1):1–3.PubMedCrossRef 4. Baichoo N, Helmann JD: Recognition of DNA by Fur: a reinterpretation of the Fur box consensus sequence. J Bacteriol 2002,184(21):5826–5832.PubMedCrossRef 5. PF477736 supplier Lavrrar JL, Christoffersen CA, McIntosh MA: Fur-DNA interactions at the bidirectional fepDGC-entS promoter region in Escherichia coli . J Mol Biol 2002,322(5):983–995.PubMedCrossRef 6. Mills SA, Marletta MA: Metal binding

characteristics and role of iron oxidation in the ferric uptake regulator from Escherichia coli . Biochemistry 2005,44(41):13553–13559.PubMedCrossRef 7. Privalle CT, Fridovich I: Iron specificity of the Fur-dependent regulation of the biosynthesis of the manganese-containing superoxide dismutase in Escherichia coli . J Biol Chem 1993,268(7):5178–5181.PubMed 8. Jacquamet L, Aberdam D, Adrait

A, Hazemann JL, Latour JM, Michaud-Soret I: X-ray absorption spectroscopy of a new zinc site in the fur protein from Escherichia coli . Biochemistry 1998,37(8):2564–2571.PubMedCrossRef 9. Althaus EW, Outten CE, Olson KE, Cao H, O’Halloran TV: The ferric uptake regulation (Fur) repressor is a zinc metalloprotein. Biochemistry 1999,38(20):6559–6569.PubMedCrossRef 10. Gaballa A, Antelmann H, Aguilar C, Khakh SK, Song KB, Smaldone GT, Helmann JD: The Bacillus subtilis iron-sparing response is mediated by a Edoxaban Fur-regulated small RNA and three small, basic proteins. Proc Natl Acad Sci USA 2008,105(33):11927–11932.PubMedCrossRef 11. Stojiljkovic I, Baumler AJ, Hantke K: Fur regulon in Gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay. J Mol Biol 1994,236(2):531–545.PubMedCrossRef 12. McHugh JP, Rodriguez-Quinones F, Abdul-Tehrani H, Svistunenko DA, Poole RK, Cooper CE, Andrews SC: Global iron-dependent gene regulation in Escherichia coli . A new mechanism for iron homeostasis. J Biol Chem 2003,278(32):29478–29486.PubMedCrossRef 13.