These protein profiles were designated FN, SN, FJ and SJ, indicating samples prepared from N16961 in fructose, N16961 in sorbitol,

JS32 in fructose, and JS32 in sorbitol fermentation medium, respectively. On the SN and FN proteome profiles, 901 and 903 spots were identified, respectively, but only 39 spots had changed in abundance, in them 27 were more abundant in N16961 cultured in sorbitol fermentation medium (SN), 12 were more abundant in check details sample of FN (Fig. 3) [also see Additional file1]. Such similarity also existed in the SJ and FJ profiles, with 34 differential spots found, 17 were more abundant in samples of SJ and FJ respectively (Fig. 3) [also see Additional file1]. All of the 73 differential protein spots were analyzed by MALDI-MS, and 71 spots significantly matched known proteins (one spot of FJ and one spot of SN were not identified)

[see Additional file1]. Sixty-two percent of the spots were identified as proteins involved in energy metabolism and central intermediary metabolism, and six spots were identified as transport/see more binding proteins. Figure 3 2-DE gels of whole cell proteins of V. cholerae strains JS32 and N16961 cultured in sorbitol and fructose fermentation media. The comparative proteins (comparison between SN/FN NVP-BSK805 ic50 and SJ/FJ) were marked and numbered on their more abundant maps. Out of 73 total differential spots identified in the SN/FN and SJ/FJ comparisons, 10 common signified potential proteins of these two comparison groups may be involved in the difference between the sorbitol and fructose metabolism

pathway: amino acid ABC transporter, perosamine synthase, malate dehydrogenase, aminotransferase NifS, heat shock protein HtpG, succinyl-CoA synthase, FIIA, glycerol kinase, pyruvate dehydrogenase, MYO10 and oxygen-insensitive NAD(P)H nitroreductase. Three of these proteins (glycerol kinase, oxygen-insensitive NAD(P)H nitroreductase, and FIIA) were more abundant in sorbitol medium. Two proteins within the identified 73 spots, the FIIA protein of PTS system and mannitol-1-P dehydrogenase (MtlD), may be involved in the transportation and transformation of sorbitol. FIIA was a common differential protein observed in the comparisons of SN/FN and SJ/FJ, both of which were more abundant in the sorbitol medium than in the fructose medium (Fig 4A). MtlD was more abundant in sorbitol than in fructose fermentation medium of the sorbitol fast-fermenting strain JS32, but was not found difference in SN/FN comparison of the sorbitol slow-fermenting strain N16961 (Fig. 4B), suggesting its potential role in the sorbitol metabolism difference between these two strains. Two different pI forms of it were also found in SJ sample (Fig. 4B).