MS and MO coded all responses A third coder (LV) coded five item

MS and MO coded all responses. A third coder (LV) coded five items independent of the other coders, to reassure reliability. Interrater reliability was considered satisfactory (k = 0.85; range = 0.25–1.0) [51]. This study was approved by the Medical Ethical Committee of Utrecht University. All participants were blind to the study aims and the condition they were assigned to via alternating enrolment. Upon registration, selleck participants completed an online questionnaire at home assessing background characteristics. The experiment took place at the Netherlands Institute for Health Services Research (NIVEL) and lasted approximately 1 h. First, participants

were welcomed and informed about the study procedures. Informed consent was obtained.

After hands and wrists were cleaned with soap, electrodes were attached to measure SCL and participants were connected to the BIOPAC equipment. Participants were instructed to not move their hands, as this may affect measurement of SCL. Before and during video-viewing, SCL was obtained. When baseline measurement was completed (4 min), participants watched one www.selleckchem.com/products/MLN-2238.html of the two videos (approximately 10 min). After video-viewing, participants were disconnected from the BIOPAC equipment and received the recall questionnaire (approximately 20 min), followed by the manipulation check questionnaire (approximately 10 min). Finally, participants were debriefed and thanked for ALOX15 their contribution. The videos contained four important time points for data-analyses. At 150 s (T1) the clinician disclosed the bad

news; this section of the consultation ended at 176 s (T2). Clinicians’ affective communication differed between 320 s (T3) and the end of the consultation (T4) in both videos. All statistical analyses were preformed at a significance level of a = 0.05 (two-tailed), using STATA 11. T-tests and chi-squared tests were used to assess differences in background characteristics. The conditions were compared using chi-squared tests, to analyse the effectiveness of the manipulation. SCL of all 50 subjects was analysed. Individual data was freed from obvious artefacts (mostly due to movement) and corrected for participants’ own baseline SCL (150 s before start of the video), using Microsoft Excel. The first part of the video (before T3) consisted of breaking the bad news and was identical in both conditions. Therefore, the effect of breaking bad news on participants’ physiological arousal was calculated for the total sample by testing the difference between mean SCL at T1 and T2, using a paired t-test. To explore the effect of clinician’s communication, all data were plotted to explore the direction of the slopes of SCL before and after T3, using Microsoft Excel.

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