Further assessments with biomechanical studies are needed to evaluate the reproducibility of these landmarks for stabilization of CCL rupture in cats.”
“Saphenous veins used as arterial grafts are exposed to arterial levels of
oxygen partial pressure (pO(2)), which are much greater than what they experience in their native environment. The object of this study is to determine the impact of exposing human saphenous veins to arterial pO(2). Saphenous veins and left internal mammary arteries from consenting patients undergoing coronary artery bypass grafting were cultured ex vivo for 2 weeks in the presence of arterial or venous pO(2) using an established organ culture model. Saphenous veins cultured with arterial pO(2) developed GSK1120212 in vitro intimal hyperplasia as evidenced by 2.8-fold greater intimal area and 5.8-fold increase in cell proliferation compared to those freshly isolated. Saphenous veins cultured at venous pO(2) or internal mammary arteries cultured at arterial pO(2) did not develop intimal hyperplasia. Intimal hyperplasia was accompanied by two markers of elevated reactive oxygen species (ROS): increased dihydroethidium associated fluorescence (4-fold, p smaller than 0.05) and increased levels of the lipid peroxidation product, 4-hydroxynonenal (10-fold, p smaller than
0.05). A functional Elacridar manufacturer role of the increased ROS saphenous veins exposed to arterial pO(2) is suggested by the observation that chronic exposure to tiron, a ROS scavenger, during the two-week culture period, blocked intimal hyperplasia. Electron paramagnetic resonance based oximetry revealed that the pO(2) in the wall of the vessel tracked that of the FK506 clinical trial atmosphere with a similar to 30 mmHg offset, thus the cells
in the vessel wall were directly exposed to variations in pO(2). Monolayer cultures of smooth muscle cells isolated from saphenous veins exhibited increased proliferation when exposed to arterial pO(2) relative to those cultured at venous pO(2). This increased proliferation was blocked by tiron. Taken together, these data suggest that exposure of human SV to arterial pO(2) stimulates IH via a ROS-dependent pathway.”
“Background: Behavioral strategies are recommended for menopausal symptoms, but little evidence exists regarding efficacy.\n\nPurpose: Describe design and methodology of a randomized controlled 3 by 2 factorial trial of yoga, exercise and omega-3 fatty acids.\n\nMethods: Women from three geographic areas with a weekly average of >= 14 hot flashes/night sweats, who met exclusion/inclusion criteria, were randomized to 12 weeks of: 1) yoga classes and daily home practice; 2) supervised, facility-based aerobic exercise training; or 3) usual activity. Women in each arm were further randomized to either omega-3 supplement or placebo.