European Journal of Cancer Prevention 20: 277-282 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.”
“The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous

studies revealed a correlation between the growth activity and dielectric property (Re[K(omega)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(omega)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To Protein Tyrosine Kinase inhibitor evaluate the activity of the cells by measuring the Re[K(omega)], the correlation between the growth activity and the Re[K(omega)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(omega)] in the cells exposed to the AC electric field were

obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(omega)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.”
“Oral Diseases (2012) 18, 741747 Objective: The aim of this study was to determine the expression of

find more N-Glycolyl GM3 (NeuGcGM3) ganglioside in oral mucosal melanomas. Materials and Methods: To assess the presence of cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) mRNA, an RT-PCR assay was performed in melanoma cell line (ME), an oral mucosal ME, and two fresh oral mucosal melanoma tissues. Expression of NeuGcGM3 ganglioside was evaluated by immunohistochemistry in 44 primary oral mucosal melanomas and 10 oral melanotic nevi. Also, the expression of NeuGcGM3 was examined in ME by immunocytochemistry. Results: We first checked the expression of CMAH in ME and two fresh oral mucosal melanoma tissues. Presence of NeuGcGM3 ganglioside was evident in 37 of 44 buy SIS3 cases (84.1%), showing a diffuse cytoplasmic and membranous staining. Patients with primary occurrence showed high levels of NeuGcGM3 ganglioside compared to patients with secondary occurrence. On the other hand, negative immunoreaction was evidenced in oral melanotic nevi. ME also presented the expression of NeuGcGM3 by immunocytochemistry. Conclusions: In this work, we for the first time evaluated the expression of 14F7 MAb immunorecognition in oral mucosal melanomas. Our results were in agreement with the assumption that NeuGcGM3 ganglioside may be considered as target for passive and active immunotherapy in oral mucosal melanomas expressing this molecule and indicate less risk of recurrence and a better prognosis.