Different forms of recombinant human L-selectin-Fc(gamma) were expressed in CHO and K-562 cells and were purified by affinity chromatography using Protein A-Sepharose. A hypersialylated form of L-selectin-Fc(gamma) was generated by culturing cells in the presence of 5 mM N-acetyl-beta-D-mannosamine. while a desialylated variant was obtained by treatment of purified L-selectin-Fc(gamma) with neuraminidase. Binding activity to the synthetic biligand SiaLe(x)-PAA-sTyr was measured by

surface plasmon resonance (SPR) technology. While hypersialylated L-selectin-Fc(gamma) showed decreased binding activity, desialylation elevated L-selectin-Fc(gamma) binding to SiaLe(x)-PAA-sTyr. The data show that sialylation of L-selectin-Fc(gamma) reduces binding activity to ligand epitopes containing sialyl Lewis x and sulfated tyrosine residues. For the production of biologically active L-selectin-Fc(gamma) conditions should be chosen Selleckchem BIX 01294 that PD0325901 favor the generation of non-sialylated or of scarcely sialylated forms of the recombinant glycoprotein. (C) 2009 Elsevier B.V. All rights reserved.”
“This case study describes a pocket salvage procedure in a patient with a device pocket infection and limited vascular access.

This case report also provides a literature review of recent studies addressing the success of pocket salvage procedures in patients not showing any signs of systemic infection. (PACE 2011; 34:e11-e13).”
“We have determined the angular distribution selleckchem of the fluorescence at 1051 nm wavelength of the Nd3+-doped monoclinic La2CaB10O19 crystal, originating from Nd3+ ions located in the regular La3+ sites, for extraordinary polarization in the XZ principal plane. We have found that the highest emission intensity does not occurs the Z-axis

direction but in a -7.1 degrees around the Y=b-axis rotated direction. With a one-pump cavity we obtained simultaneous lasing at 1051 and 1069 nm in X and Z-polarizations from Nd3+ ions located in La3+ and Ca2+ sites, respectively. In the active Q-switch regime the two laser pulses are always simultaneous. With a two-pump setup based on an optical bifurcated fiber we obtained also simultaneous lasing at 1051 and 1069 nm. The relative power of the two laser channels is tuneable with the help of a beam-splitter. An adequate value of the relative pump power of the two channels leads to simultaneous pulses in the active Q-switch regime. (C) 2010 American Institute of Physics. [doi:10.1063/1.3503237]“
“A high amylase producing Bacillus subtilis WD 161 was used in a co-culture with Clostridium butylicum TISTR 1032 to enhance acetone-butanol-ethanol (ABE) production from starch. The mixed culture of C butylicum TISTR 1032 and B. subtilis WD 161 without anaerobic pretreatment by reducing agent and N(2) flushing increased amylase activity 10 folds and enhanced ABE production 5.