5. The distribution of genes into COGs functional categories is presented in Table 5. Figure 6 Graphical circular map of the M. massiliensis selleck chemicals MEK162 strain NP3T chromosome. From the outside in: the outer two circles show open reading frames oriented in the forward and reverse (colored by COG categories) directions, respectively. The third circle displays … Table 4 Nucleotide content and gene count levels of the genome Table 5 Number of genes associated with the 25 general COG functional categories Comparison with the genomes from M. elsdenii, Megasphaera species, Veillonella dispar, V. parvula and Anaeroglobus geminatus The draft genome of M. massiliensis strain NP3T (2.66 Mb) has a larger size than that of M. elsdenii (2.47 Mb), V. parvula (2.13 Mb), V. dispar (2.12 Mb), A. geminatus (1.79 Mb) and M.

micronuciformis (1.77 Mb) respectively. M. massiliensis has a lower G + C content (50.2%) than M. elsdenii (52.8%) but higher than V. parvula, V. dispar, M. micronuciformis and A. geminatus (38.6, 38.8, 46.8 and 48.7%, respectively). M. massiliensis (2,516) has more predicted protein-coding genes than M. elsdenii, A. geminatus, V. dispar, V. parvula, Inhibitors,Modulators,Libraries and M. micronuciformis (2,219, 2,148, 1,954, 1,844 and 1,774, respectively) (Table 6). In addition, M. massiliensis shared a mean genomic sequence similarity of 81.84, 69.44, 63.68, 62.92 and 70.27% with M. elsdenii, M. micronuciformis, V. dispar, V. parvula and A. geminatus respectively (Table 6). Table 6 Orthologous gene comparison and average nucleotide identity of M. massiliensis with other compared genomes ? M.

massiliensis harbors two intact bacteriophages. Based on PHAST results, phage 1 of M. massiliensis was most closely related to Clostridium phage phi CD119 whereas phage 2 was most similar to Bacillus Inhibitors,Modulators,Libraries phage BCJA1c. Conclusion On the basis of phenotypic, phylogenetic and genomic analyses, we formally propose the creation of Megasphaera massiliensis sp. nov. that contains the strain NP3T. This bacterial strain has been found in Marseille, France. Description of Megasphaera massiliensis sp. nov. Megasphaera massiliensis (mas.il.ien��sis. L. gen. fem. n. massiliensis, of Massilia, the Latin name of Marseille where was cultivated strain NP3T). It has been isolated from Inhibitors,Modulators,Libraries the Inhibitors,Modulators,Libraries feces of a 32-year-old HIV-infected French patient. Colonies were smooth and transparent with 0.5 to 1 mm in diameter on blood-enriched Columbia agar.

Optimal growth is only achieved anaerobically and grows between 30 and 45��C, with optimal growth observed at 37��C. The strain is a Gram-negative, non-endospore forming, non motile coccobacillus. Inhibitors,Modulators,Libraries Positive for ��-glucosidase, ��-glucosidase, potassium gluconate, potassium 5-cetogluconate, aesculin, salicine, N-acetylglucosamine, and arbutine production. Positive for L-arabinose, D-ribose, D-xylose, D-galactose, D-glucose, D-fructose, D-mannose, Drug_discovery L-rhamnose, D-mannitol, D-sorbitol, D-celiobiose, D-maltose, D-lactose, D-trehalose, gentiobiose, L-fucose and D-arabitol fermentation.