The telomers were characterized by size-exclusion chromatography and MALDI-TOF-MS. In initial surface characterization experiments, the prepared telomers were attached to the inner surface of fused silica capillaries by radical initiated addition to vinyl groups, and the electro-osmotic flow (EOF) in the prepared capillaries was determined in a capillary electrophoresis set-up. The EOF measurements verified surface grafting. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 117: 2781-2789, 2010″
“The nuclear pore complex (NPC) is the gate to the nucleus. Recent determination of the configuration of proteins in the yeast NPC at similar to 5 nm resolution

permits us to study the NPC global dynamics using coarse-grained Smoothened Agonist inhibitor structural models. We investigate these large-scale motions by using an extended elastic network model (ENM) formalism applied to several coarse-grained representations of the NPC. Two types of collective motions (global modes) are predicted by the ENMs to be intrinsically

favored by the NPC architecture: global bending and extension/contraction from circular to elliptical shapes. These motions are shown to be robust against tested variations in the representation of the NPC, and are largely captured by a simple model of a toroid with axially varying mass density. We demonstrate that spoke multiplicity significantly affects the accessible number of symmetric low-energy modes of motion; learn more the NPC-like toroidal structures composed Bcl2 inhibitor of 8 spokes have access to highly cooperative symmetric motions that are inaccessible to toroids composed of 7 or 9 spokes. The analysis reveals modes of motion that may facilitate macromolecular transport through the NPC, consistent with previous experimental observations.”
“Lipid-based drug delivery systems show great potential for enhancing oral bioavailability but have not been broadly applied, largely

due to lack of general formulation guidance. In the previous studies, three different formulations including anionic SLNs, cationic SLNs, and liposomes were investigated and significantly enhanced the oral bioavailability of N-3-o-toluyl-fluorouracil (TFu) compared with its aqueous suspension, which indicated their high potential as oral delivery carriers. In order to define which formulation is worthy of being further researched and developed, the studies on Caco-2 cell model and rat intestine were investigated. In both studies of crossing Caco-2 cell monolayers and the single-pass intestinal perfusion (SPIP) in rat, SLNs exhibited much more capability to enhance transport of TFu than liposomes. More specifically, in cell study, the P-app values of cationic SLNs (p < 0.01) and anionic SLNs (p < 0.05) were significantly higher than liposomes. Especially the cationic SLNs present the most effective capacity.