However, several species of butterflyfishes and damselfishes were recorded picking at the remains, mostly from Day 2 to Day 4. Glynn (1984) suggested that exposure of internal organs can considerably increase the likelihood of attacks by a broader array of predators or scavengers and reported that internal tissues of A. planci were acceptable as food to fishes even if it is not part of their ordinary diet. Finally, there were no incidences of coral

disease or partial mortality recorded on individually tagged coral colonies within the following month after the injections. Oxbile provides a relatively effective medium to control A. planci, Selleckchem SB203580 requiring only a single injection, preferably at the base of one arm. At 8 g l−1 of Bile Salts No. 3 (Oxoid®), A. planci die rapidly regardless of the site of injection, though it is possible that when injected into

the oral disk, the sea star can rapidly expel the oxbile through the stomach and mouth. Thus, A. planci should be injected at the base of an arm in the polian vesicle area were the coelomic fluid is stored. Bile salts disrupt cell membranes and induce osmotic shock through their detergent action ( Rolo et al., 2004). Thus, injection of oxbile in this area will ensure a rapid distribution of the solution throughout the sea star and will affect directly the organ in charge of maintaining hydrostatic pressure ( Lawrence, 2001). The resulting death of A. planci is caused by cell membrane

and mitochondria damage (by creation of channels) coupled with a dramatic immune response to the tissue damaged caused BIBW2992 in vivo by bile salts ( Rivera-Posada et al., 2011 and Grand et al., 2014). The benefit of this new method was extremely apparent following the first field trial, whereby divers from the Association of Marine Park Tourism Operators (AMPTO), killed A. planci at a rate of 5–6 sea stars per minute using single injections of bile salts, compared to just 1 sea star per minute with sodium bisulfate. Moreover, there was no flow-on effects of this chemical, even among fishes (Arothron spp.) that consume large Ibrutinib clinical trial quantities of A. planci remains following injection of higher doses of bile salts, either in aquaria or in the field. Given rapid mortality and no apparent increase in concentrations of bacteria among tissues of sea stars killed using oxbile, the risk of direct transmission of disease (e.g., to corals) appears very minimal. Similarly, the risk of toxicity from excess oxbile consumption by organisms that consume A. planci remains (e.g., Arothron spp.) is very low, especially among vertebrates that naturally produce and can readily excrete bile. In addition, the low quantity of bile (0.08 mg per sea star) used to control A. planci ( Table 3) will be rapidly degraded by marine bacteria that use bile as energy source ( Maneerat et al., 2005 and Birkeland, 1990).