All preparations showed antioxidant activity, but the ethanolic one (EME) was significantly more active than the aqueous one (AME) as hydrogen or electron donors with
SC50 values between 1.45 to 6.31 mu g GAE/mL and 7.38 to 64.77 mu g GAE/mL, respectively. The aqueous extraction was significantly more active than EME on superoxide and hydroxyl radical scavenging. Polyphenols showed a dose-response relationship (R-2>0.90) with antioxidant capacity in the decoction and the alcoholic beverage. The maceration showed an inhibitory effect on lipoxygenase (LOX) activity with an inhibitory concentration (IC50) value of 183.80 BAY 57-1293 mouse mu g gallic acid equivalents (GAE)/mL but the decoction did not. On the other hand, extracts did not show any mutagenic effect. Therefore, mistol fruits consumption could be encouraged not only for its functional properties, but also because of the positive ecological impact of preserving biological diversity through the exploitation of native natural resources in a regional economy.
(C) 2011 Elsevier Ltd. All rights reserved.”
“BACKGROUND: Only about 15% of donor lungs are considered suitable for transplantation (LTx). Ex vivo lung perfusion (EVLP) has been developed as a method to reassess and repair damaged lungs. Selleckchem Avapritinib We report our experience with EVLP in non-acceptable donor lungs and evaluate its ability to recondition these lungs.
METHODS: We studied lungs from 16 brain-dead 8-Bromo-cAMP donors rejected for LTx. After harvesting, the lungs were stored at 4 degrees C for 10 hours and subjected to normothermic EVLP with Steen Solution (Vitro life, Goteborg, Sweden) for 60 minutes. For functional evaluation, the following variables were assessed: partial pressure of arterial oxygen (Pao(2)), pulmonary vascular resistance (PVR), and lung compliance (LC). For histologic assessment, lung biopsy was done before harvest and after EVLP. Tissue samples were examined under light microscopy. To detect and quantify apoptosis, terminal deoxynucleotide transferase-mediated deoxy uridine
triphosphate nick-end labeling assay was used.
RESULTS: Thirteen lima donors were refused for having impaired lung function. The mean Pao(2) obtained in the organ donor at the referring hospital was 193.7 mm Hg and rose to 489 mm Hg after EVLP. During EVLP, the mean PVR was 652.5 dynes/sec/cm(5) and the mean LC was 48 ml/cm H2O. There was no significant difference between the mean Lung Injury Score before harvest and after EVLP. There was a trend toward a reduction in the median number of apoptotic cells after EVLP.
CONCLUSIONS: EVLP improved lung function (oxygenation capacity) of organs considered unsuitable for transplantation. Lung tissue structure did not deteriorate even after 1 hour of normothermic perfusion. J Heart Lung Transplant 2012;31:305-9 (C) 2012 International Society for Heart and Lung Transplantation. All rights reserved.